Detalle de: Crystallization of the CHAP domain of the endolysin from Staphylococcus aureus bacteriophage K.
| Title | Crystallization of the CHAP domain of the endolysin from Staphylococcus aureus bacteriophage K. |
| Abstract |
CHAP(K) is the N-terminal cysteine, histidine-dependent amidohydrolase/peptidase domain (CHAP domain) of the Staphylococcus aureus bacteriophage K endolysin LysK. It is formed from the first 165 residues of LysK and functions by cleaving specific peptidoglycan peptide bonds, causing bacterial lysis. CHAP(K) can lyse S. aureus when applied exogenously, making it a good candidate for the treatment of multidrug-resistant Staphylococcus aureus infections. Here, the crystallization of CHAP(K) and the collection of native and derivative data to high resolution, which allowed structure solution, are reported. The structure may help to elucidate the mechanism of action and in the design of chimeric proteins or mutants with improved antibacterial activity. |
| Apa Reference | Sanz-Gaitero, M., Keary, R., Garcia-Doval, C., Coffey, A., & van Raaij, M. J. (2013). Crystallization of the CHAP domain of the endolysin fromStaphylococcus aureusbacteriophage K. Acta Crystallographica Section F Structural Biology and Crystallization Communications, 69(12), 1393–1396. 10.1107/s1744309113030133 |
| Doi | 10.1107/S1744309113030133 |
| Publication Date | 29 Nov 2013 |
| Publication Type | Article |
| Researcher | Mark van Raaij |
| Ontology | Mutation Analysis and design, Structure-function relationship, Viromics |
| Research Groups | Structural Biology of Virus Proteins |
| Tools |