Detalle de: The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics
| Title | The SGN VIGS tool: user-friendly software to design virus-induced gene silencing (VIGS) constructs for functional genomics |
| Abstract |
Virus-induced gene silencing (VIGS) is a fast and powerful method to study gene function in plants (Burch-Smith et al., 2004). It is based on plant defense mechanisms against viral gene replication and allows high-throughput silencing of genes of interest (Senthil-Kumar and Mysore, 2014). The molecular mechanisms involved in post-transcriptional gene silencing (PTGS) have been studied intensively, and its steps are well known. The silencing process begins with the recognition through Dicer-like ribonucleases (DCL) of double-stranded RNA (dsRNA) that is generated during viral replication. |
| Apa Reference | Fernandez-Pozo, N., Rosli, H. G., Martin, G. B., & Mueller, L. A. (2015). The SGN VIGS Tool: User-Friendly Software to Design Virus-Induced Gene Silencing (VIGS) Constructs for Functional Genomics. Molecular Plant, 8(3), 486–488. 10.1016/j.molp.2014.11.024 |
| Doi | 10.1016/j.molp.2014.11.024 |
| Publication Date | Mar 2015 |
| Publication Type | Article |
| Researcher | Noé Fernández Pozo |
| Ontology | Genomics, Functional genomics |
| Research Groups | Breeding and developmental biology of subtropical fruit |
| Tools |