Detalle de: The development of genetic and molecular markers to register and commercialize Penicillium rubens (formerly Penicillium oxalicum) strain 212 as a biocontrol agent

Summary P enicillium oxalicum strain 212 ( PO 212) is an effective biocontrol agent ( BCA ) against a large number of economically important fungal plant pathogens. For successful registration as a BCA in E urope, PO 212 must be accurately identified. In this report, we describe the use of classical genetic and molecular markers to characterize and identify PO 212 in order to understand its ecological role in the environment or host. We successfully generated pyrimidine ( pyr ‐) auxotrophic mutants. In addition we also designed specific oligonucleotides for the pyrF gene at their untranslated regions for rapid and reliable identification and classification of strains of P . oxalicum and P . rubens , formerly P . chrysogenum . Using these DNA ‐based technologies, we found that PO 212 is a strain of P . rubens , and is not a strain of P . oxalicum . This work presents PO 212 as the unique P . rubens strain to be described as a BCA and the information contained here serves for its registration and commercialization in Europe.