Detalle de: IN-VITRO BINDING OF THE 2-FINGER REPRESSOR CREA TO SEVERAL CONSENSUS AND NONCONSENSUS SITES AT THE IPNA UPSTREAM REGION IS CONTEXT-DEPENDENT
| Title | IN-VITRO BINDING OF THE 2-FINGER REPRESSOR CREA TO SEVERAL CONSENSUS AND NONCONSENSUS SITES AT THE IPNA UPSTREAM REGION IS CONTEXT-DEPENDENT |
| Abstract |
The two zinc‐fingers of the Aspergillus nidulans repressor CreA recognize the consensus hexanucleotide 5'‐SYGGRG‐3'. We have determined all the CreA binding sites in a ∼2 kb region upstream the ipnA gene. Our analysis shows that (i) CreA binds to certain consensus sites in a context‐dependent manner; (ii) five non‐consensus 6‐bp sequences are also recognized by CreA; this non‐canonical binding correlates with the presence of a second, neighbouring CreA binding site, suggesting that recognition of two linked sites stabilizes CreA binding. Our results suggest that the binding possibilities of CreA might be more complex than originally envisaged. |
| Apa Reference | Espeso, E. A., & Peñalva, M. A. (1994). In vitro binding of the two‐finger repressor CreA to several consensus and non‐consensus sites at the ipnA upstream region is context dependent. FEBS Letters, 342(1), 43–48. Portico. 10.1016/0014-5793(94)80581-4 |
| Doi | 10.1016/0014-5793(94)80581-4 |
| Publication Date | 28 Mar 1994 |
| Publication Type | Article |
| Researcher | Eduardo Antonio Espeso Fernández |
| Ontology | Model organisms, Molecular Interactions/Docking |
| Research Groups | Aspergillus Cell Biology |
| Tools |