Detalle de: A genome annotation-driven approach to cloning the human ORFeome
| Title | A genome annotation-driven approach to cloning the human ORFeome |
| Abstract |
We have developed a systematic approach to generating cDNA clones containing full-length open reading frames (ORFs), exploiting knowledge of gene structure from genomic sequence. Each ORF was amplified by PCR from a pool of primary cDNAs, cloned and confirmed by sequencing. We obtained clones representing 70% of genes on human chromosome 22, whereas searching available cDNA clone collections found at best 48% from a single collection and 60% for all collections combined. |
| Apa Reference | Collins, J.E., Wright, C.L., Edwards, C.A., Davis, M.P., Grinham, J.A., Cole, C.G., Goward, M.E., Aguado, B., Mallya, M., Mokrab, Y., Huckle, E.J., Beare, D.M. & Dunham, I. (2004). A genome annotation-driven approach to cloning the human ORFeome. Genome Biology, 5(10). 10.1186/gb-2004-5-10-r84 |
| Doi | 10.1186/gb-2004-5-10-r84 |
| Publication Date | 30 Sep 2004 |
| Publication Type | Article |
| Researcher | Begoña Aguado Orea † |
| Ontology | Genomics, Genomic Annotation |
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