Detalle de: Monoclonal antibodies against sporangia and spores of Marteilla sp. (Protozoa: Ascetospora)

Digestive glands of mussels Mytilus edulis from Brittany, France, infected with Marteilia sp.(Ascetospora) were used to purify the parasite.A modification of a previously used purification protocol increased punfication efficiency, permitting sporangial primordia and sporangia of Marteilia sp. to be obtained.Mouse (Balb/c) monoclonal antibodies were generated against this parasite.From the fusion, 26 rnonoclonal antibodies against Marteilia sp. were obtained.Antibodies from 6 clones reacted only with Marteilia sp. cells and not with normal host tissues.Four of these antibodies (1/1-3, 311-1, 4/1-1 and 6/2-3) reacted with the sporangia wall and two with the spore cytoplasm (9/1-1 and 12/51), Antibodies cross-reacted with A4arteilia refringens from Mytilus galloprovincialis obtained in the Ria de Vigo, Spain.