Detalle de: Algorithmic robustness to preferred orientations in single particle analysis by CryoEM
| Title | Algorithmic robustness to preferred orientations in single particle analysis by CryoEM |
| Abstract |
The presence of preferred orientations in single particle analysis (SPA) by cryo-Electron Microscopy (cryoEM) is currently one of the hurdles preventing many structural analyses from yielding high-resolution structures. Although the existence of preferred orientations is mostly related to the grid preparation, in this technical note, we show that some image processing algorithms used for angular assignment and three-dimensional (3D) reconstruction are more robust than others to these detrimental conditions. We exemplify this argument with three different data sets in which the presence of preferred orientations hindered achieving a 3D reconstruction without artifacts or, even worse, a 3D reconstruction could never be achieved. |
| Apa Reference | Sorzano, C., Semchonok, D., Lin, S., Lo, Y., Vilas, J., Jiménez-Moreno, A., Gragera, M., Vacca, S., Maluenda, D., Martínez, M., Ramírez-Aportela, E., Melero, R., Cuervo, A., Conesa, J., Conesa, P., Losana, P., Caño, L.d., de la Morena, J.J., Fonseca, Y., Sánchez-García, R., Strelak, D., Fernández-Giménez, E., de Isidro, F., Herreros, D., Kastritis, P., Marabini, R., Bruce, B. & Carazo, J. (2021). Algorithmic robustness to preferred orientations in single particle analysis by CryoEM. Journal of Structural Biology, 213(1), 107695. 10.1016/j.jsb.2020.107695 |
| Doi | 10.1016/j.jsb.2020.107695 |
| Publication Date | Mar 2021 |
| Publication Type | Article |
| Researcher | Jose María Salvador Carazo Garcia |
| Ontology | Experimental structure determination, Image analysis, Cryo-electron microscopy |
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