Detalle de: XTEND: Extending the depth of field in cryo soft X-ray tomography
| Title | XTEND: Extending the depth of field in cryo soft X-ray tomography |
| Abstract |
We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters. |
| Apa Reference | Otón, J., Pereiro, E., Conesa, J.J., Chichón, F.J., Luque, D., Rodríguez, J.M., Pérez-Berná, A.J., Sorzano, C.O.S., Klukowska, J., Herman, G.T., Vargas, J., Marabini, R., Carrascosa, J.L. & Carazo, J.M. (2017). XTEND: Extending the depth of field in cryo soft X-ray tomography. Scientific Reports, 7(1). 10.1038/srep45808 |
| Doi | 10.1038/srep45808 |
| Publication Date | 4 Apr 2017 |
| Publication Type | Article |
| Researcher | Jose María Salvador Carazo Garcia |
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