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Detalle de: 3-D Reconstruction of 2-D Crystals in Real Space

Title 3-D Reconstruction of 2-D Crystals in Real Space
Abstract

A new algorithm for three-dimensional reconstruction of two-dimensional crystals from projections is presented, and its applicability to biological macromolecules imaged using transmission electron microscopy (TEM) is investigated. Its main departures from the traditional approach is that it works in real space, rather than in Fourier space, and it is iterative. This has the advantage of making it convenient to introduce additional constraints (such as the support of the function to be reconstructed, which may be known from alternative measurements) and has the potential of more accurately modeling the TEM image formation process. Phantom experiments indicate the superiority of the new approach even without the introduction of constraints in addition to the projection data.

Apa Reference Marabini, R., Sorzano, C., Matej, S., Fernandez, J., Carazo, J. & Herman, G. (2004). 3-D Reconstruction of 2-D Crystals in Real Space. IEEE Transactions on Image Processing, 13(4), 549-561. 10.1109/tip.2003.822620
Doi 10.1109/tip.2003.822620
Publication Date Apr 2004
Publication Type Article
Researcher Jose María Salvador Carazo Garcia
Ontology Experimental structure determination
Research Groups
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