Detalle de: Glucose-Tolerant Expression of Trichoderma longibrachiatum Endoglucanase I, an Enzyme Suitable for Use in Wine Production
| Title | Glucose-Tolerant Expression of Trichoderma longibrachiatum Endoglucanase I, an Enzyme Suitable for Use in Wine Production |
| Abstract |
A Trichoderma longibrachiatum transformant has been constructed constitutively expressing the homologous egl1 gene under the control of the Aspergillus nidulans glyceraldehyde phosphate dehydrogenase gene (gpdA) promoter. Consequently, the egl1 gene can be expressed in glucose-containing media. The egl1 product (EGL1) has been purified from such a medium. The physicochemical and kinetic properties of EGL1, which has both endoglucanase and xylanase activities, have been determined. Analysis of the effects of certain enological factors such as temperature, pH, and glucose, ethanol, and SO2 concentrations on the enzyme's activities indicates that it can be used in wine production. |
| Apa Reference | Ganga, A., González-Candelas, L., Ramón, D. & Pérez-González, J.A. (1997). Glucose-Tolerant Expression of Trichoderma longibrachiatum Endoglucanase I, an Enzyme Suitable for Use in Wine Production. Journal of Agricultural and Food Chemistry, 45(6), 2359-2362. 10.1021/JF960904X |
| Doi | 10.1021/JF960904X |
| Publication Date | 1 Jun 1997 |
| Publication Type | Article |
| Researcher | Luis Gonzalez-Candelas |
| Ontology | Model organisms, Regulatory biology |
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