Detalle de: A protocol to extract cell-type-specific signatures from differentially expressed genes in bulk-tissue RNA-seq
| Title | A protocol to extract cell-type-specific signatures from differentially expressed genes in bulk-tissue RNA-seq |
| Abstract |
Bulk-tissue RNA-seq is widely used to dissect variation in gene expression levels across tissues and under different experimental conditions. Here, we introduce a protocol that leverages existing single-cell expression data to deconvolve patterns of cell-type-specific gene expression in differentially expressed gene lists from highly heterogeneous tissue. We apply this protocol to interrogate cell-type-specific gene expression and variation in cell type composition between the distinct sublayers of the hippocampal CA1 region of the brain in a rodent model of epilepsy. For complete details on the use and execution of this protocol, please refer to Cid et al. (2021). |
| Apa Reference | Marquez-Galera, A., de la Prida, L.M. & Lopez-Atalaya, J.P. (2022). A protocol to extract cell-type-specific signatures from differentially expressed genes in bulk-tissue RNA-seq. STAR Protocols, 3(1), 101121. 10.1016/j.xpro.2022.101121 |
| Doi | 10.1016/j.xpro.2022.101121 |
| Publication Date | Mar 2022 |
| Publication Type | Article |
| Researcher | Jose P. López-Atalaya Martínez |
| Ontology | Transcriptomics, RNA Sequencing (RNA-seq), Expression Profiling, Single-cell omics |
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