Detalle de: Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans
| Title | Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans |
| Abstract |
We describe an automated method to isolate mutant Caenorhabditis elegans that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately. |
| Apa Reference | Doitsidou, M., Flames, N., Lee, A.C., Boyanov, A. & Hobert, O. (2008). Automated screening for mutants affecting dopaminergic-neuron specification in C. elegans. Nature Methods, 5(10), 869-872. 10.1038/nmeth.1250 |
| Doi | 10.1038/nmeth.1250 |
| Publication Date | 31 Aug 2008 |
| Publication Type | Article |
| Researcher | Nuria Flames |
| Ontology | Model organisms, Mutation Analysis and design |
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