Detalle de: Single-cell preparation and scRNA-Seq data analysis v1
| Title | Single-cell preparation and scRNA-Seq data analysis v1 |
| Abstract |
The Epithelial to Mesenchymal transition (EMT) triggers cell plasticity in embryonic development, adult injured tissues, and cancer. Here, we provide a step-by-step protocol to prepare single-cell RNAseq libraries frommousemodels of renal fibrosis and breast cancer. We further provide a detailed workflowfordifferent analyses used tocharacterise theEMTprogrammeas assessed fromsingle-celltranscriptomes, to reconstruct EMT trajectories, and to predict theEMT-associatedmolecular programmes in fibrosis and cancer. |
| Apa Reference | Kass Youssef, K., NARWADE, N. & Nieto, A. (2024). Single-cell preparation and scRNA-Seq data analysis v1. 10.17504/protocols.io.eq2lyw9qwvx9/v1 |
| Doi | 10.17504/protocols.io.eq2lyw9qwvx9/v1 |
| Publication Date | 25 Jul 2024 |
| Publication Type | Preprint |
| Researcher | Nitin Narwade |
| Ontology | Transcriptomics, RNA Sequencing (RNA-seq), Data Analysis, Single-cell omics, Single-cell (scRNA-seq), Development |
| Research Groups | |
| Tools |