Detalle de: Crystallographic ab initio protein structure solution below atomic resolution
| Title | Crystallographic ab initio protein structure solution below atomic resolution |
| Abstract |
Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small á-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A. |
| Apa Reference | Rodríguez, D.D., Grosse, C., Himmel, S., González, C., de Ilarduya, I.M., Becker, S., Sheldrick, G.M. & Usón, I. (2009). Crystallographic ab initio protein structure solution below atomic resolution. Nature Methods, 6(9), 651-653. 10.1038/nmeth.1365 |
| Doi | 10.1038/nmeth.1365 |
| Publication Date | 16 Aug 2009 |
| Publication Type | Software |
| Researcher | Isabel Uson Finkenzeller |
| Ontology | Structural bioinformatics, Experimental structure determination |
| Research Groups | |
| Tools | ARCIMBOLDO |