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Detalle de: S -nitroso- and nitro- proteomes in the olive ( Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS

Title S -nitroso- and nitro- proteomes in the olive ( Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS
Abstract

The data presented here are related to the research article entitled "Generation of nitric oxide by olive ( Olea europaea L.) pollen during in vitro germination and assessment of the S -nitroso- and nitro-proteomes by computational predictive methods" doi:10.1016/j.niox.2017.06.005 (Jimenez-Quesada et al., 2017) [1]. Predicted cysteine S -nitrosylation and Tyr-nitration sites in proteins derived from a de novo assembled and annotated pollen transcriptome from olive tree ( Olea europaea L.) were obtained after using well-established predictive tools in silico . Predictions were performed using both default and highly restrictive thresholds. Numerous gene products identified with these characteristics are listed here. An experimental validation of the data, consisting in nano-LC-MS (Liquid Chromatography-Mass Spectrometry) determination of olive pollen proteins after immunoprecipitation with antibodies to anti- S -nitrosoCys and anti-3-NT (NitroTyrosine) allowed identification of numerous proteins subjected to these two post-translational modifications, which are listed here together with information regarding their cross-presence among the predictions.

Apa Reference Carmona, R., Jimenez-Quesada, M.J., Lima-Cabello, E., Traverso, J.Á., Castro, A.J., Claros, M.G. & de Dios Alché, J. (2017). S -nitroso- and nitro- proteomes in the olive ( Olea europaea L.) pollen. Predictive versus experimental data by nano-LC-MS. Data in Brief, 15, 474-477. 10.1016/j.dib.2017.09.058
Doi 10.1016/j.dib.2017.09.058
Publication Date Dec 2017
Publication Type Article
Ontology Transcriptomics, Genome Assembly, Computational models and simulations, Proteomics
Researchers M. Gonzalo Claros Díaz
Research Groups
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