Detalle de: Metabolic heterogeneity of monoclonal bacterial cells as a biotechnological tool to produce natural compounds (MENTHOL)

Coordinator and Co-PI CSIC: Juan Nogales.

MENTHOL aimed to change current paradigms of biotechnological production of complex plant natural compounds, specifically isoprenoids such as limonene, menthol, and p-cymene. MENTHOL main innovation lies in the synthetic decoupling of the complex biosynthetic pathways of these compounds into a distributed catalysis within monoclonal heterogeneous bacterial populations. To achieve such ambitious goal, in this project we applied cutting-edge systems and synthetic biology tools to engineer programmable metabolic heterogeneity inside a monoclonal population of Pseudomonas putida, thus promoting the division of labour during the production of limonene, menthol, and p-cymene using waste cooking oils as economic and reliable renewable feedstock.