Detalle de: Electron Tomography of Biological Specimens

This chapter addresses the issue of three-dimensional reconstruction of biological macromolecules from electron microscopy images. First, the electron microscope device, the micrographs as specimen projections and the projection collection geometry are discussed. After that, a brief introduction to the problems involved in sample preparation and observation is made. In a third section the different three-dimensional reconstruction methods are presented, and finally, the chapter finishes with an example in which a protein is reconstructed starting from micrographs and ending with a nice and very informative volume.