Detalle de: Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes
| Title | Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes |
| Abstract |
In the present work, we describe the sequence, organization and expression of histone H4 genes in the protozoan parasite Leishmania infantum. The predicted L. infantum histone H4 is a polypeptide of 100 amino acids with a molecular mass of 11.5 kDa. Comparison of the amino acid sequence of Leishmania histone H4 with the rest of histone H4 sequences indicates that this is the most divergent sequence reported to date. The genomic distribution analysis of histone H4 genes indicates that there must be up to seven gene copies. A single size-class histone H4 mRNA of 0.6 kb was detected, whose level dramatically decreases from logarithmic to stationary phase. However, the Leishmania histone H4 mRNAs do not decrease in abundance following treatment with inhibitors of DNA synthesis, suggesting a regulation by a replication-independent mechanism. |
| Apa Reference | Soto, M., Quijada, L., Alonso, C., & Requena, J. M. (1997). Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes1Note: Nucleotide sequence data reported in this paper have been submitted to the EMBL/GenBank/DDBJ databases with the accession numbers Y13915 (cDNA LiH4-1) and Y13916 (cDNA LiH4-2).1. Molecular and Biochemical Parasitology, 90(2), 439–447. 10.1016/s0166-6851(97)00178-3 |
| Doi | 10.1016/S0166-6851(97)00178-3 |
| Publication Date | Dec 1997 |
| Publication Type | Article |
| Researcher | José María Requena Rolanía |
| Ontology | Genomics, Drug Discovery and design, Histone structure and regulation |
| Research Groups | Regulation of gene expression in Leishmania |
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