Researcher

José María Requena Rolanía

Regulation of gene expression in Leishmania · CBM

Biography

Profesor Titular de Universidad (Associate Professor) at the Department of Molecular Biology of the Universidad Autónoma de Madrid (UAM) and researcher at the Centro de Biología Molecular Severo Ochoa (CBMSO) since 1998, teaching Clinical Microbiology in the Degree in Biochemistry. Developing a repository with updated information on the genomes, transcriptomes and proteomes of the Leishmania parasite: https://jmrequenajmr.wixsite.com/leish-esp (Description curated from publicly available data.)

Profile

Institution	CBM
Position	Associate Professor (Profesor Titular de Universidad)
Home page	https://www.linkedin.com/in/jose-m-requena-893a902a/
Contact email	jm.requena@uam.es
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Research

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Publications 97 entries

Assembly of a Large Collection of Maxicircle Sequences and Their Usefulness for Leishmania Taxonomy and Strain Typing Article · 15 Jun 2022
The Astonishing Large Family of HSP40/DnaJ Proteins Existing in Leishmania Article · 23 Apr 2022
Live attenuated vaccines, a favorable strategy to provide long-term immunity against protozoan diseases Article · Apr 2022
A dataset of proteins associated with Trypanosoma cruzi LYT1 mRNAs Article · Apr 2022
Protective Efficacy in a Hamster Model of a Multivalent Vaccine for Human Visceral Leishmaniasis (MuLeVaClin) Consisting of the KMP11, LEISH-F3+, and LJL143 Antigens in Virosomes, Plus GLA-SE Adjuvant Article · 29 Oct 2021

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Inoculation of the Leishmania infantum HSP70-II Null Mutant Induces Long-Term Protection against L. amazonensis Infection in BALB/c Mice Article · 12 Feb 2021
The Experimental Proteome of Leishmania infantum Promastigote and Its Usefulness for Improving Gene Annotations Article · 2 Sep 2020
Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice Article · 23 Mar 2020
Leishmania Mitochondrial Genomes: Maxicircle Structure and Heterogeneity of Minicircles Article · 26 Sep 2019
Analysis by RNA-seq of transcriptomic changes elicited by heat shock in Leishmania major Article · 6 May 2019
Identificación de proteínas reguladoras de la expresión génica en tripanosomátidos Article · 26 Dec 2018
Characterization of the mRNA untranslated regions [UTR] of the Trypanosoma cruzi LYT1 isoforms derived by alternative trans-splicing Article · 25 Sep 2018
Vaccination with a Leishmania infantum HSP70-II null mutant confers long-term protective immunity against Leishmania major infection in two mice models Article · 30 May 2017
Dataset for distribution of SIDER2 elements in the Leishmania major genome and transcriptome Article · Apr 2017
Genomic cartography and proposal of nomenclature for the repeated, interspersed elements of the Leishmania major SIDER2 family and identification of SIDER2-containing transcripts Article · Mar 2017
A putative Leishmania DNA polymerase theta protects the parasite against oxidative damage Article · 29 Apr 2016
Resequencing and assembly of seven complex loci to improve the Leishmania major (Friedlin strain) reference genome Article · 8 Feb 2016
Molecular Chaperones of Leishmania: Central Players in Many Stress-Related and -Unrelated Physiological Processes Article · 2015
Leishmania braziliensis replication protein A subunit 1: Molecular modelling, protein expression and analysis of its affinity for both DNA and RNA Article · Dec 2014
Identification of proteins interacting with HSP70 mRNAs in Leishmania Braziliensis Article · Dec 2013
Evolution and species discrimination according to the Leishmania heat-shock protein 20 gene. Article · Aug 2013
Alpha tubulin genes from Leishmania braziliensis: genomic organization, gene structure and insights on their expression. Article · 6 Jul 2013
Gimatecan and other camptothecin derivatives poison Leishmania DNA-topoisomerase IB leading to a strong leishmanicidal effect Article · May 2013
The transcriptome of Leishmania major in the axenic promastigote stage: transcript annotation and relative expression levels by RNA-seq. Article · 4 Apr 2013
Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis Article · 29 Nov 2012
Indotecan (LMP400) and AM13-55: Two novel indenoisoquinolines show potential for treating visceral leishmaniasis Article · Oct 2012
Sequence analysis of the 3'-untranslated region of HSP70 (type I) genes in the genus Leishmania: its usefulness as a molecular marker for species identification. Article · 28 Apr 2012
Immunity to visceral leishmaniasis. Article · 2012
Identification of the HSP70-II gene in Leishmania braziliensis HSP70 locus: genomic organization and UTRs characterization. Article · 26 Aug 2011
Leishmania infantum HSP70-II null mutant as candidate vaccine against leishmaniasis: a preliminary evaluation. Article · 27 Jul 2011
Lights and shadows on gene organization and regulation of gene expression in Leishmania. Article · 2011
Evidence of RNA editing in Leishmania braziliensis promastigotes. Article · 4 Dec 2010
The Leishmania infantum PUF proteins are targets of the humoral response during visceral leishmaniasis. Article · 2010
Immunogenicity of HSP-70, KMP-11 and PFR-2 leishmanial antigens in the experimental model of canine visceral leishmaniasis Article · Mar 2008
Effects of the disruption of the HSP70-II gene on the growth, morphology, and virulence of Leishmania infantum promastigotes Article · 2008
The SIDER2 elements, interspersed repeated sequences that populate the Leishmania genomes, constitute subfamilies showing chromosomal proximity relationship. Article · 2008
Pitfalls of the CAT reporter gene for analyzing translational regulation in Leishmania. Article · 5 Aug 2007
A postgenomic view of the heat shock proteins in kinetoplastids. Article · Jul 2007
Genomic organization and expression of the HSP70 locus in New and Old World Leishmania species Article · 23 Oct 2006
Immunohistological features of visceral leishmaniasis in BALB/c mice Article · 10 Feb 2006
The translational efficiencies of the two Leishmania infantum HSP70 mRNAs, differing in their 3'-untranslated regions, are affected by shifts in the temperature of growth through different mechanisms. Article · Oct 2005
Genomic DNA macroarrays as a tool for analysis of gene expression in Leishmania. Article · Sep 2005
Vaccination with a plasmid DNA cocktail encoding the nucleosomal histones of Leishmania confers protection against murine cutaneous leishmaniosis. Article · Sep 2004
Recent advances in vaccines for leishmaniasis. Article · Sep 2004
The expression of HSP83 genes in Leishmania infantum is affected by temperature and by stage-differentiation and is regulated at the levels of mRNA stability and translation. Article · 3 Jun 2004
Adjuvant guided polarization of the immune humoral response against a protective multicomponent antigenic protein (Q) from Leishmania infantum. A CpG + Q mix protects Balb/c mice from infection Article · Jun 2004
Cell-cycle-dependent translation of histone mRNAs is the key control point for regulation of histone biosynthesis in Leishmania infantum. Article · 1 May 2004
Molecular and functional characterization of adenylate kinase 2 gene from Leishmania donovani Article · 16 Oct 2003
Identification of a gene in Leishmania infantum encoding a protein that contains a SP-RING/MIZ zinc finger domain Article · 1 Oct 2003
Leishmania infantum possesses a complex family of histone H2A genes: Structural characterization and analysis of expression Article · Aug 2003
High-efficiency plating method for Leishmania infantum. Article · Aug 2003
A Leishmania infantum multi-component antigenic protein mixed with live BCG confers protection to dogs experimentally infected with L. infantum Article · Mar 2003
Conserved nucleotides surrounding the trans-splicing acceptor site and the translation initiation codon in Leishmania genes. Article · Jan 2003
Antigenic properties of the Leishmania infantum GRP94 and mapping of linear B-cell epitopes. Article · 1 Mar 2002
Analysis of the adjuvant effect of recombinant Leishmania infantum Hsp83 protein as a tool for vaccination Article · Mar 2001
The Leishmania infantum acidic ribosomal protein LiP2a induces a prominent humoral response in vivo and stimulates cell proliferation in vitro and interferon-gamma (IFN-γ) production by murine splenocytes Article · Nov 2000
Leishmania infantum: Gene cloning of the GRP94 homologue, its expression as recombinant protein, and analysis of antigenicity Article · Oct 2000
Immune and clinical parameters associated with Leishmania infantum infection in the golden hamster model Article · Oct 2000
Identification of a putative regulatory element in the 3'-untranslated region that controls expression of HSP70 in Leishmania infantum Article · Sep 2000
Evolutionarily conserved proteins as prominent immunogens during Leishmania infections Article · Jun 2000
Histone synthesis in Leishmania infantum is tightly linked to DNA replication by a translational control Article · 8 Feb 2000
Immunostimulatory properties of the Leishmania infantum heat shock proteins HSP70 and HSP83 Article · Dec 1999
Phage recovery by electroporation of naked DNA into host cells avoids the use of packaging extracts Article · Feb 1999
Antigenicity of the Leishmania infantum histones H2B and H4 during canine viscerocutaneous leishmaniasis Article · Feb 1999
Analysis of the humoral immune response against total and recombinant antigens of Leishmania infantum: Correlation with disease progression in canine experimental leishmaniasis Article · Feb 1999
Analysis of the antigenic properties of the L. infantum Hsp70: Design of synthetic peptides for specific serodiagnosis of human leishmaniasis Article · Oct 1998
Polymerase chain reaction procedure to detect Trypanosoma cruzi in blood samples from chronic chagasic patients Article · Mar 1998
Cloning, sequencing, and expression of the PSA genes from Leishmania infantum Article · Jan 1998
Molecular cloning and analysis of expression of the Leishmania infantum histone H4 genes Article · Dec 1997
Characterization of an interspersed repetitive DNA element in the genome of Trypanosoma cruzi Article · Dec 1997
Analysis of post-transcriptional regulation operating on transcription products of the tandemly linked Leishmania infantum hsp70 genes Article · Feb 1997
Cloning of genes and expression and antigenicity analysis of the Leishmania infantum KMP-11 protein Article · Jan 1997
A region containing repeated elements is associated with transcriptional termination of Leishmania infantum ribosomal RNA genes Article · Jan 1997
During canine leishmaniasis a protein belonging to the 83-kDa heat-shock protein family elicits a strong humoral response Article · Sep 1996
Genomic repetitive DNA elements of Trypanosoma cruzi Article · Jul 1996
Mapping of the linear antigenic determinants from the Leishmania infantum histone H2A recognized by sera from dogs with leishmaniasis Article · Dec 1995
Identification of the Leishmania infantum P0 ribosomal protein epitope in canine visceral leishmaniasis Article · Nov 1995
Assignment of genes to Leishmania infantum chromosomes: karyotype and ploidy Article · Jun 1995
Molecular karyotype of clone CL Brener chosen for the Trypanosoma cruzi Genome Project Article · May 1995
The Leishmania infantum histone H3 possesses an extremely divergent N-terminal domain Article · Oct 1994
Characterization of a short interspersed reiterated DNA sequence of Trypanosoma cruzi located at the 3′-end of a poly(A)+ transcript Article · Sep 1994
Cytoplasmic-Nuclear Translocation of the HSP70 Protein during Environmental Stress in Trypanosoma cruzi Article · Nov 1993
Isolation, characterization and analysis of the expression of the Leishmania ribosomal PO protein genes Article · Oct 1993
Binding of Pt-pentamidine to nucleosomal DNA. Studies of the antiproliferative activity of the drug against human cancer cells Article · Oct 1993
Mapping of antigenic determinants of the T. cruzi HSP70 in chagasic and healthy individuals Article · Aug 1993
Bacteriophage λ: long-term stored stocks ready for lysis Article · Jan 1993
Regulation of hsp70 expression in Trypanosoma cruzi by temperature and growth phase Article · Jul 1992
Molecular characterization of a Leishmania donovani infantum antigen identified as histone H2A Article · Apr 1992
Characterization of a highly repeated interspersed DNA sequence of Trypanosoma cruzi: its potential use in diagnosis and strain classification Article · Apr 1992
A potential Z-DNA-forming sequence is located between two transcription units alternatively expressed during development of Drosophila hydei Article · Jan 1991
The mRNA coding for the nucleosomal protein H2A of Leishmania is polyadenylated and has stem-loops at the 3′ end Article · 1991
DNA binding properties and antileukemic (L1210) activity of a Pt-pentamidine complex Article · 1991
The heat shock protein hsp70 binds in vivo to subregions 2-48BC and 3-58D of the polytene chromosomes of Drosophila hydei Article · Sep 1990
Sequence and expression of the Drosophila phenylalanine hydroxylase mRNA Article · Jan 1990
Molecular cloning of a Drosophila potential Z-DNA forming sequence hybridizing in situ to a developmentally regulated subdivision of the polytene chromosomes Article · 1989
Complete nucleotide sequence of the hsp70 gene of T.cruzi Article · 1989
A head-to-tail tandem organization of hsp70 genes in Trypanosoma cruzi Article · 1988

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