Detalle de: PYRUVATE-DEHYDROGENASE DEPHOSPHORYLATION IN RAT-BRAIN SYNAPTOSOMES AND MITOCHONDRIA - EVIDENCE FOR A CALCIUM-MEDIATED EFFECT IN RESPONSE TO DEPOLARIZATION, AND VARIATIONS DUE TO AGING
| Title | PYRUVATE-DEHYDROGENASE DEPHOSPHORYLATION IN RAT-BRAIN SYNAPTOSOMES AND MITOCHONDRIA - EVIDENCE FOR A CALCIUM-MEDIATED EFFECT IN RESPONSE TO DEPOLARIZATION, AND VARIATIONS DUE TO AGING |
| Abstract |
The phosphorylation state of P42, the phosphorylated, catalytically inactive, alpha-subunit of pyruvate dehydrogenase (PDH), decreased markedly (42.4%) in response to K(+)-depolarization of synaptosomes. The dephosphorylation was rapid (5-15 s), calcium-dependent and could also be observed in isolated mitochondria exposed to a rise in extramitochondrial calcium, suggesting that P42 dephosphorylation may act as a calcium sensor in the mitochondrial matrix. The depolarization-dependent dephosphorylation rate of P42 was decreased in synaptosomes derived from 24-month-old animals with respect to 3-month-old adults. The relevance of these results in terms of PDH activation during ageing is discussed. |
| Apa Reference | Bogónez, E., Gómez-Puertas, P., & Satrústegui, J. (1992). Pyruvate dehydrogenase dephosphorylation in rat brain synaptosomes and mitochondria: Evidence for a calcium-mediated effect in response to depolarization, and variations due to ageing. Neuroscience Letters, 142(2), 123–127. 10.1016/0304-3940(92)90354-a |
| Doi | 10.1016/0304-3940(92)90354-A |
| Publication Date | Aug 1992 |
| Publication Type | Article |
| Researcher | Paulino Gomez-Puertas |
| Ontology | |
| Research Groups | Molecular Modeling Group |
| Tools |