Detalle de: In Vivo Chaperone‐Assisted Folding of α ‐1,6‐Fucosyltransferase from Rhizobium sp.

Three is not a crowd: Coexpression of the chaperonins GroEL/GroES with the α-1,6-fucosyltransferase from Rhizobium sp. allows a 10-fold increase in production of soluble and active fucosyltransferase. The His-tagged fucosyltransferase can be purified and immobilized on Ni2+-IDA-agarose gel in only one step (see Scheme). The immobilization leads to an important stabilization of the recombinant enzyme, which allows the preparation of a robust biocatalyst for the synthesis of oligosaccharides.