Detalle de: Holzapfeliella saturejae sp. nov. isolated from flowers of winter savoury Satureja montana L.

A novel strain of the genus Holzapfeliella , named He02 T , was isolated from flowers of Satureja montana L. in a survey for lactic acid bacteria associated with wild and cultivated plants in the metropolitan area of Valencia, Spain. Partial 16S rRNA gene sequencing revealed a similarity of 99% to Holzapfeliella floricola DSM 23037 T =Ryu1-2 T . Strain He02 T cells are Gram-stain-positive, catalase-negative non-motile rods, usually occurring in pairs. Cells show a pale yellow pigmentation when pelleted. As H. floricola , strain He02 T utilized a narrow range of carbohydrates, namely, glucose and fructose, homofermentatively. However, genome sequencing and estimation of average nucleotide identity (ANI) revealed an ANI value of 87.44 with H. floricola DSM 23037 T , the only H. floricola strain sequenced to date. A value of 30.5% for digital DNA–DNA hybridization was estimated with the Type Strain Genome Server tool when He02 T was compared with strain DSM 23037 T . These results indicate that strain He02 T constitutes a novel species, for which the name Holzapfeliella saturejae sp. nov. with He02 T (=CECT 31001 T =DSM 117324 T =CCM 9395 T ) as type strain is proposed.