Detalle de: Relative expression of Slug, RhoB, and HNK‐1 in the cranial neural crest of the early chicken embryo

The neural crest constitutes a complex population of cells that originates at the edges of the neural plate of vertebrate embryos and gives rise to a high diversity of tissues and cell types. Molecular markers are very useful to identify cell populations, and in the case of the neural crest at early stages, many of them have been described. Here, we show a series of chicken embryos double labeled for several of the most commonly used crest markers that evidence the existence of different subpopulations. Slug is a very good marker for premigratory and early migratory cranial neural crest, RhoB labels delaminating cells and the very early migratory population, and the HNK‐1 epitope is acquired in the migratory crest cells at a distance from the neural tube, with a significant proportion of the Slug ‐expressing migratory cells negative for HNK‐1. The existence of these crest subpopulations should be considered when analyzing both wild‐type embryos and the phenotype of experimentally manipulated chick embryos. Developmental Dynamics 229:136–139, 2004. © 2003 Wiley–Liss, Inc.