Detalle de: Correction for Quer at al., High-Resolution Hepatitis C Virus Subtyping Using NS5B Deep Sequencing and Phylogeny, an Alternative to Current Methods
| Title | Correction for Quer at al., High-Resolution Hepatitis C Virus Subtyping Using NS5B Deep Sequencing and Phylogeny, an Alternative to Current Methods |
| Abstract |
HepatitisCvirus (HCV)is classified into seven major genotypesand67 subtypes. Recent studies haveshownthat inHCVgenotype 1-infected patients, response rates to regimens containingdirect-acting antivirals(DAAs)are subtype dependent. Currently available genotypingmethods have limited subtyping accuracy.Wehave evaluated theperformanceof adeep-sequencing-basedHCVsubtyping assay, developed for the 454/GS-Junior platform, incomparisonwith thoseof twocommercial assays (VersantHCVgenotype 2.0andAbbott Real-timeHCVGenotype II)andusingdirectNS5Bsequencing as a gold standard (direct sequencing), in 114 clinical specimenspreviously tested by first-generation hybridization assay (82 genotype 1 and32 with uninterpretable results). Phylogenetic analysis of deep-sequencing reads matched subtype 1 callingbypopulation Sanger sequencing (69%1b,31%1a) in81 specimensandidentified amixed-subtype infection (1b/3a/1a) inone sample. Similarly,amongthe 32previously indeterminate specimens, identical genotypeandsubtype results were obtained by directanddeep sequencing inall but four samples with dual infection. In contrast, both VersantHCVGenotype 2.0andAbbott Real-timeHCVGenotype II failed subtype 1 calling in13 (16%)samples eachandwere unable to identify theHCVgenotype and/or subtype inmore thanhalfof the nongenotype 1 samples.Weconcluded that deep sequencing ismore efficient forHCVsubtyping than currently available methods andallows qualitative identificationofmixed infectionsandmay bemorehelpfulwith respect toinforming treatment strategies withnewDAA-containing regimens across allHCVsubtypes |
| Apa Reference | Quer, J., Gregori, J., Rodríguez-Frias, F., Buti, M., Madejon, A., Perez-del-Pulgar, S., Garcia-Cehic, D., Casillas, R., Blasi, M., Homs, M., Tabernero, D., Alvarez-Tejado, M., Muñoz, J.M., Cubero, M., Caballero, A., delCampo, J.A., Domingo, E., Belmonte, I., Nieto, L., Lens, S., Muñoz-de-Rueda, P., Sanz-Cameno, P., Sauleda, S., Bes, M., Gomez, J., Briones, C., Perales, C., Sheldon, J., Castells, L., Viladomiu, L., Salmeron, J., Ruiz-Extremera, A., Quiles-Pérez, R., Moreno-Otero, R., López-Rodríguez, R., Allende, H., Romero-Gómez, M., Guardia, J., Esteban, R., Garcia-Samaniego, J., Forns, X. & Esteban, J.I. (2016). Correction for Quer at al., High-Resolution Hepatitis C Virus Subtyping Using NS5B Deep Sequencing and Phylogeny, an Alternative to Current Methods. Journal of Clinical Microbiology, 54(7), 1933-1933. 10.1128/JCM.01007-16 |
| Doi | 10.1128/JCM.01007-16 |
| Publication Date | Jul 2016 |
| Publication Type | Article |
| Researcher | Carlos Briones Llorente |
| Ontology | Population genomics, Phylogenetic Analysis |
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| Tools |