Detalle de: Sequential dual site-selective protein labelling enabled by lysine modification
| Title | Sequential dual site-selective protein labelling enabled by lysine modification |
| Abstract |
Methods that allow for chemical site-selective dual protein modification are scarce. Here, we provide proof-of-concept for the orthogonality and compatibility of a method for regioselective lysine modification with strategies for protein modification at cysteine and genetically encoded ketone-tagged amino acids. This sequential, orthogonal approach was applied to albumin and a therapeutic antibody to create functional dual site-selectively labelled proteins. |
| Apa Reference | Matos, M.J., Brown, L., Bernardim, B., Guerreiro, A., Jiménez-Osés, G. & Bernardes, G.J. (2020). Sequential dual site-selective protein labelling enabled by lysine modification. Bioorganic & Medicinal Chemistry, 28(22), 115783. 10.1016/j.bmc.2020.115783 |
| Doi | 10.1016/j.bmc.2020.115783 |
| Publication Date | Nov 2020 |
| Publication Type | Article |
| Researcher | Gonzalo Jiménez-Osés |
| Ontology | |
| Research Groups | |
| Tools |