Detalle de: Cell‐Penetrating Peptides Containing Fluorescent d ‐Cysteines

A series of fluorescent d ‐cysteines (Cys) has been synthesized and their optical properties were studied. The key synthetic step is the highly diastereoselective 1,4‐conjugate addition of aryl thiols to a chiral bicyclic dehydroalanine recently developed by our group. This reaction is fast at room temperature and proceeds with total chemo‐ and stereoselectivity. The Michael adducts were easily transformed into the corresponding amino acids to study their optical properties and, in some selected cases, into the corresponding N ‐Fmoc‐ d ‐cysteine derivatives to be used in solid‐phase peptide synthesis (SPPS). To further demonstrate the utility of these non‐natural Cys‐derived fluorescent amino acids, the coumaryl and dansyl derivatives were incorporated into cell‐penetrating peptide sequences through standard SPPS and their optical properties were studied in different cell lines. The internalization of these fluorescent peptides was monitored by fluorescence microscopy.