Golden Standard
Golden Standard (GS) is a Type IIS assembly method underpinned by the Standard European Vector Architecture. GS unlocks modular cloning applications for most bacteria, and delivers combinatorial multi-part assembly to create genetic circuits of up to twenty transcription units (TUs). Reliance on MoClo syntax renders GS fully compatible with many existing tools and it sets the path towards efficient reusability of available part libraries and assembled TUs.
GS provides a computational pipeline for parts characterization and a dedicated web-portal including a repository of parts, vectors, and Wizard and Setup tools that guide users in designing constructs.
Basic Information
| Repository | http://sysbiol.cnb.csic.es/GoldenStandard/home.php |
| Documentation | |
| Main publication (DOI) | 10.1093/nar/gkad758 view |
| Contact email | jnogales@cnb.csic.es |
| Authors/Maintainers | Juan Nogales |
| Type of application | Web-service |
| Programming languages | |
| Keywords | Computational Methods, Applications of Computational Biology, Model organisms, Systems Biology, Pathway analysis, Non-model organisms, Microbial communities |
Technical details
| Input file formats | |
| Output file formats | |
| Software compatibility | Linux, Mac, Windows |
| Hardware requirements | |
| Type of containerization | None |
| Wrapper type | |
| Compatible tools | MoClo, GoldenBraid, CIDAR, Mobius assembly, Midas, CyanoGate, uLoop, JUMP, The Marburg Collection, SEVA (Standard European Vector Architecture) |
| Funding resource | |
| Funding support |
Statistics
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| Citations (last 12 months) | <100 (~14 as of 15/10/2026) |
| Citations (since first release) | <100 (60 as of 15/10/2026) |
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